Fz2 but not Ryk recruits Dvl. (A) Schematic of mosaic injection approach. At the 8–16-cell stage, one cell was injected with Wnt5b-IRES-nls-EGFP RNA while an adjacent cell was injected with Dvl-EGFP RNA alone or combined with Fz2-mCherry RNA, Fz2-mCherry RNA + Ryk MO, or Ryk-mCherry RNA. (B–D) Fz-mCherry (B) when co-injected with Dvl-GFP (C) is concentrated in discrete membrane domains and colocalized with Dvl-EGFP (D, arrowheads) when adjacent to a Wnt5b-expressing cell (C and D; nuclear EGFP). (E–G) The pattern of Fz-mCherry (E) colocalized with Dvl-GFP (F and G) is not affected by co-injection of Ryk (G). Arrowheads in G mark the location of Dvl-EGFP. (H–J) Ryk-mCherry (H) when co-injected with Dvl-GFP (I) does not colocalize with Dvl-EGFP (J). Note that Ryk-expressing cells develop lamellipodia-like protrusions (H), and Dvl-EGFP is largely located in cytoplasmic areas that are Ryk-mCherry negative (J, arrowheads). (K) Quantification of the ratio of colocalized Dvl-EGFP versus total Dvl-EGFP area. Column height represents mean ratio in percentage. Error bars represent standard error of seven samples in each experiment. One-way analysis of variance and Tukey’s honestly significant difference test were used (*, P < 0.01). Bar, 10 µm.