Ryk deficiency leads to directional cell migration defects in a Wnt5b-dependent manner. (A–D) Assessment of cell migration. Donor cells separately labeled with FITC (green) or Texas red (red) were transplanted into the center of the lateral mesoderm at the germ ring margin (A). Uninjected host with control (Ctrl.) MO–injected (red and green) donor cells (B); uninjected host with control MO–injected (green) and Ryk MO–injected (red) donor cells (C); Wnt5b MO–injected host with control MO–injected (green) and Ryk MO–injected (red) donor cells (D). (E) Quantification of the mean distance between green and red tracers. 10 embryos were scored for each group, and error bars represent standard deviation. One-way analysis of variance and Tukey’s honestly significant difference test were used to determine statistical significance. Bar, 250 µm.