Wnt5b and Ryk show synergistic effects in zebrafish CE. (A–D) In situ hybridization with combined myoD, krox20, and pax2a probes. Dorsal view, anterior to the left of control (A) and low-dose Wnt5b MO (0.5 pmol; B)–, low-dose Ryk MO (1.0 pmol; C)–, and combined Wnt5b MO (0.25 pmol) and Ryk MO (0.5 pmol; D)–injected embryos. (E) Quantification of CE index defects. Bar, 150 µm.