NPA fibroblasts are deficient in plasma membrane resealing after SLO wounding. (A) FACS quantification of PI staining in normal control or NPA fibroblasts after SLO permeabilization. Without Ca²⁺ (Mg²⁺; middle), both groups of cells remained permeabilized; with Ca²⁺, normal fibroblasts resealed more efficiently (right). Percentages correspond to resealed (PI negative) cells in the gated region (dashed lines). (B) Images of PI-stained normal or NPA fibroblasts permeabilized by SLO. NPA fibroblasts showed increased levels of PI staining after wounding in the presence of Ca²⁺ when compared with normal control fibroblasts. The results shown in this figure are representative of several independent experiments. Bars, 20 µm.