Figure 4.
Figure 4. Inhibition of ASM activity impairs restoration of plasma membrane integrity. (A) FACS quantification of PI staining in nontreated or DPA-treated cells wounded by SLO permeabilization. (B) FACS quantification of PI staining in nontreated or DPA-treated cells, mechanically wounded by scraping from the dish. Without Ca2+ (Mg2+), both groups of cells remained permeabilized; with Ca2+, DPA-treated cells resealed less efficiently than nontreated cells. Percentages correspond to resealed (PI negative) cells in the gated region (dashed lines). The results shown in this figure are representative of several independent experiments.

Inhibition of ASM activity impairs restoration of plasma membrane integrity. (A) FACS quantification of PI staining in nontreated or DPA-treated cells wounded by SLO permeabilization. (B) FACS quantification of PI staining in nontreated or DPA-treated cells, mechanically wounded by scraping from the dish. Without Ca2+ (Mg2+), both groups of cells remained permeabilized; with Ca2+, DPA-treated cells resealed less efficiently than nontreated cells. Percentages correspond to resealed (PI negative) cells in the gated region (dashed lines). The results shown in this figure are representative of several independent experiments.

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