Figure 9.
Figure 9. Laminin–integrin signaling activates the LL5/CLASP/EB1–microtubule anchoring pathway. (A and B) Analysis of EB1-GFP comet numbers at the basal cell cortex in cells treated with the indicated siRNAs. The cells were maintained on collagen IV–coated glass substrates to retain siRNA-treated cells. (A) Representative images are shown. (B) The number of EB1-GFP comets in the 8 × 8–µm boxed area was counted. For each condition, n > 31. The results are presented as means ± SEM (*, P < 0.01 vs. the mock control). (C) Microtubule organization in MCF-10A cells estimated from the observations obtained in this study. LL5s attach microtubule ends to the basal cortex and suppress their dynamicity. Without LL5 activities, the microtubule-stabilizing activity at the basal cortex is reduced, and once microtubules reach the basal surface, they remain dynamic. (D) Schematic diagram of the molecular link between laminin-5 and microtubule plus ends. Bar, 5 µm.

Laminin–integrin signaling activates the LL5/CLASP/EB1–microtubule anchoring pathway. (A and B) Analysis of EB1-GFP comet numbers at the basal cell cortex in cells treated with the indicated siRNAs. The cells were maintained on collagen IV–coated glass substrates to retain siRNA-treated cells. (A) Representative images are shown. (B) The number of EB1-GFP comets in the 8 × 8–µm boxed area was counted. For each condition, n > 31. The results are presented as means ± SEM (*, P < 0.01 vs. the mock control). (C) Microtubule organization in MCF-10A cells estimated from the observations obtained in this study. LL5s attach microtubule ends to the basal cortex and suppress their dynamicity. Without LL5 activities, the microtubule-stabilizing activity at the basal cortex is reduced, and once microtubules reach the basal surface, they remain dynamic. (D) Schematic diagram of the molecular link between laminin-5 and microtubule plus ends. Bar, 5 µm.

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