p150^glued accumulates at spindle poles of aurora A RNAi-deficient S2 cells and aurora A mutant neuroblasts. (A) Control S2 cells (top) were fixed and stained for tubulin (red), p150^glued (green), and DNA (blue). The mitotic phases are displayed at the top. p150^glued localization at spindle poles is clearly detected during prophase and cytokinesis, whereas the signal is very weak during prometaphase and metaphase. In parallel, ∼60% of aurora A–depleted cells (bottom) exhibit p150^glued accumulation at spindle poles (arrows). (B) Wild-type (left) or aurora A^e209/Df(3R)T61 (right) mutant neuroblasts were fixed and stained for tubulin (green; monochrome in middle), p150^glued (red; monochrome in bottom), and DNA (blue). In control metaphase cells, p150^glued is faintly detected along spindle fibers. In aurora A mutant neuroblasts, p150^glued accumulates on spindle fibers and spindle poles (arrowheads). Bars, 10 µm.