During hemocyte contact repulsion, microtubule arms between colliding cells transiently interact. (a) Time-lapse imaging of stage 15 hemocytes expressing GFP-Moesin revealed how a single cell (asterisks) persistently collides (arrows) with neighbors and is immediately repelled from them. Time is shown in minutes. (b) Hemocytes expressing both GFP-Moesin (actin) and mCherry-CLIP170 (microtubules) indicate how the microtubule arms between contacting hemocytes align before cells retract from one another (arrows). (c) Time-lapse imaging of colliding hemocytes revealed that the lamellar interaction (arrowheads) precedes microtubule alignment (arrows). Microtubules were pseudocolored purple in the merged images. Time is shown in seconds. (d) Turn angles of colliding and noncolliding hemocytes after a 3-min time period revealed a greater change of direction after cell collision (P < 0.01; χ2 test). Bars, 10 µm.
