The N-terminal domain of human CLIP170 reveals both stable and dynamic microtubules in Drosophila hemocytes. (a) GFP-Moesin (actin) and two copies of mCherry-CLIP170 (microtubules) were expressed in stage 15 hemocytes and live-imaged by confocal microscopy. Microtubules surrounded the cell body (arrow), with some penetrating into the lamellae (arrowheads). (b) Time-lapse imaging of a hemocyte expressing two copies of GFP-CLIP170 revealed microtubules extending (asterisks), pausing (plus signs), buckling (arrows), and reextending (arrowheads) within the lamellae. Brackets indicate the length of extension from 0 to 30 s. (c) A single copy of GFP-CLIP170 labeled the tips of growing microtubules (arrows). (d) Time-lapse series of the microtubule filament highlighted in c (boxed area) revealed cycles of microtubule growth, pausing, catastrophe, and regrowth. Bars, 10 µm.