The Dam1 complex tracks plus ends independently of Bim1p in vivo. (A) Schematic illustration of the live cell imaging strategy (Tanaka et al., 2005). (B) Time-lapse live cell microscopy showing plus end tracking of the Dam1 complex (arrowheads) during phases of microtubule growth and shrinkage (Videos 1 and 2). Insets show higher magnification views of the indicated panel (120 s). (C) Western blot analysis showing depletion of Bim1p-3xHA in a Dam1-3xGFP, mCherry-tubulin background probed with an anti-HA antibody. (D) Still image from a video depicting microtubule plus end localization of the Dam1 complex (arrowheads) in the absence of the +TIP Bim1p (Video 3). Bars, 2 µm.