Pull-downs of pericentrin splice isoforms. (A–C) Volcano plots representing results of the label-free pull-downs of GFP-tagged pericentrin^short and pericentrin^long. The logarithmic ratio of protein intensities in the pericentrin/HeLa (A and B) and pericentrin^long/pericentrin^short (C) pull-downs were plotted against negative logarithmic p-values of the t test performed from triplicates. (A and B) The hyperbolic curve separates specific pericentrin-interacting proteins marked in black (red dotted line) from background (blue dots). (C) Proteins binding specifically to either form of pericentrin are marked in black. The dotted line represents the ratio of pericentrin^long/pericentrin^short. (D) Plotted relative intensities of all peptides identified from pericentrin^short (blue) and pericentrin^long (red). The N-terminal part of the protein was identified in both pull-downs, whereas there is a stretch of ∼500 amino acids unique to the pericentrin^short form (green box). The C terminus was deleted in pericentrin^short, and therefore, peptides from this region were only identified in the pull-downs of pericentrin^long (brown box).