Mitotic spindle localization interdependencies of CLTC, TACC3, and GTSE1 by RNAi and after aurora A inhibition. (A) Live imaging of mitotic cells expressing GFP-tagged CLTC, TACC3, or GTSE1 after RNAi (72 h). GTSE1 is mislocalized after CLTC or TACC3 RNAi, TACC3 is mislocalized after CLTC, but not GTSE1 RNAi, and CLTC is not mislocalized by either TACC3 or GTSE1 RNAi. Two images of representative cells are shown for each condition. (B) Live imaging of mitotic cells expressing GFP-tagged CLTC, TACC3, or GTSE1 after treatment with the aurora A kinase inhibitor MLN8054. Inhibition of aurora A activity mislocalizes GTSE1 but not CLTC from the mitotic spindle. Two images of representative cells are shown for each condition. CON, control. Bars, 10 µm.