Figure 2.
Figure 2. Nuclear accumulation of cyclin B1–Cdk1 depends on Cdk activity. (A) HeLa cells coexpressing the cyclin B1–Cdk1 activity FRET sensor and cyclin B1–mCherry were recorded at one image every 2 min as cells entered mitosis. After cyclin B1 began to move into the nucleus, 300 nM Cdk1/2 inhibitor III was added. The quantification curves correspond to the cell displayed. Note the immediate decrease in the nuclear accumulation rate of cyclin B1 just after addition of the Cdk inhibitor before its reexport ∼3 min later. (B) Cells analyzed as in A were treated with 5 µM aurora B inhibitor ZM447439 in late G2 phase (n = 6). Bars, 10 µm.

Nuclear accumulation of cyclin B1–Cdk1 depends on Cdk activity. (A) HeLa cells coexpressing the cyclin B1–Cdk1 activity FRET sensor and cyclin B1–mCherry were recorded at one image every 2 min as cells entered mitosis. After cyclin B1 began to move into the nucleus, 300 nM Cdk1/2 inhibitor III was added. The quantification curves correspond to the cell displayed. Note the immediate decrease in the nuclear accumulation rate of cyclin B1 just after addition of the Cdk inhibitor before its reexport ∼3 min later. (B) Cells analyzed as in A were treated with 5 µM aurora B inhibitor ZM447439 in late G2 phase (n = 6). Bars, 10 µm.

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