Figure 5. TNF induces colocalization of... | Rockefeller University Press

Figure 5.

$Figure 5. TNF induces colocalization of occludin and caveolin-1 but not clathrin heavy chain. Wild-type mice were injected with vehicle or TNF, and jejunum harvested at the times indicated. Sections were labeled for occludin (green), nuclei (blue), and clathrin heavy chain, caveolin-1, or EEA1 (all red). Apical–basal-oriented and orthogonal sections are shown. Morphometric analysis of the fraction of occludin-containing vesicles that also contain caveolin-1 (red squares), clathrin heavy chain (green circles), and EEA1 (blue triangles) is shown below the micrographs. Error bars indicate mean ± SEM. Bars, 10 µm.$

TNF induces colocalization of occludin and caveolin-1 but not clathrin heavy chain. Wild-type mice were injected with vehicle or TNF, and jejunum harvested at the times indicated. Sections were labeled for occludin (green), nuclei (blue), and clathrin heavy chain, caveolin-1, or EEA1 (all red). Apical–basal-oriented and orthogonal sections are shown. Morphometric analysis of the fraction of occludin-containing vesicles that also contain caveolin-1 (red squares), clathrin heavy chain (green circles), and EEA1 (blue triangles) is shown below the micrographs. Error bars indicate mean ± SEM. Bars, 10 µm.