Aip1 localizes to sites of active actin filament disassembly. (A) Two-color imaging showing CFP-cofilin and Aip1-GFP localization in sla2Δ cells expressing Abp1-mRFP. Quantification of normalized fluorescence intensities (fluor int) along the length of the actin tails moving from the outside of the cell toward the cell interior is presented in the graph. (B) Single frames from simultaneous two-color imaging of Abp1-mRFP and Aip1-GFP. The maturation of a single actin patch over time is shown by the montage, where each frame represents a 1-s exposure. The mean temporal delay in Aip1-GFP at the patch is indicated (n = 51). A kymograph illustrates the spatiotemporal relationship between Aip1-GFP and Abp1-mRFP. The kymograph is oriented so that the cell exterior is on the top and the cell interior is oriented downward. dfc, distance from cortex.