Lat A–insensitive actin assembly at endocytic sites in wild-type cells. Single frames from simultaneous two-color imaging of Abp1-mRFP and Sla1-GFP in cells treated with 200 µM lat A are shown at the indicated times relative to lat A treatment. Kymographs show the spatiotemporal dynamics of actin assembly events at endocytic sites at early, intermediate, or late time points after lat A addition. The kymographs are oriented so that the cell exterior is on the top and the cell interior is oriented downward. dfc, distance from cortex.