Actin assembly continues in sla2Δ cells treated with lat A. (A) A single frame from time-lapse imaging of Abp1-mRFP in sla2Δ cells. Kymographs show irregular actin assembly indicated by the oblique dark lines that traverse the actin tails. The details of these fiduciary marks are highlighted by a kymograph in which black and white have been inverted and by a schematic in which the red lines highlight each actin flux fiduciary mark. The kymographs are oriented so that the cell exterior is on the top and the cell interior is oriented downward. dfc, distance from cortex. (B) Single frames from time-lapse imaging of Abp1-mRFP in sla2Δ cells treated with 400 µM lat A. A kymograph shows the rapid, directional disassembly toward the cell cortex. The schematic highlights actin flux fiduciary marks as well as the actin tail shrinkage fiduciary marks. (C) Quantification of actin flux rates and tail shrinkage rates in sla2Δ cells treated with a range of concentrations of lat A. 16 cells were quantified before and after 50 µM lat A treatment, 13 cells for 100 µM, 14 cells for 200 µM, and 14 cells for 400 µM lat A. Three measurements for before and after lat A treatment were averaged per cell. Error bars represent SD.