Figure 1.
Figure 1. ARL-13/Arl13b is enriched in proximal regions of ciliary axonemes. (A) ARL-13::GFP localizes almost exclusively to the proximal region (MSs; m) of amphid/phasmid cilia and male tail ray cilia. Although localizing to all regions of AWB cilia, ARL-13 is enriched in proximal regions and observed at the membrane of distal fans (arrow). IFT markers (CHE-2 and -11) label amphid/phasmid channel MSs and DSs (d) and RnA/B male tail ray cilia. Kymographs, from time-lapse videos, show no IFT movement for ARL-13::GFP. Arrowheads indicate RnA/B cilia, and insets show high magnification images of RnA/B cilia. (B) Arl13b is enriched in the proximal region of MDCKII cilia. Images after immunostaining for endogenous Arl13b, acetylated α-tubulin (ciliary axoneme), and γ-tubulin (basal body) are shown. Bars: (A, left and right) 3 µm; (A, middle) 10 µm; (A, insets) 1 µm; (B) 5 µm.

ARL-13/Arl13b is enriched in proximal regions of ciliary axonemes. (A) ARL-13::GFP localizes almost exclusively to the proximal region (MSs; m) of amphid/phasmid cilia and male tail ray cilia. Although localizing to all regions of AWB cilia, ARL-13 is enriched in proximal regions and observed at the membrane of distal fans (arrow). IFT markers (CHE-2 and -11) label amphid/phasmid channel MSs and DSs (d) and RnA/B male tail ray cilia. Kymographs, from time-lapse videos, show no IFT movement for ARL-13::GFP. Arrowheads indicate RnA/B cilia, and insets show high magnification images of RnA/B cilia. (B) Arl13b is enriched in the proximal region of MDCKII cilia. Images after immunostaining for endogenous Arl13b, acetylated α-tubulin (ciliary axoneme), and γ-tubulin (basal body) are shown. Bars: (A, left and right) 3 µm; (A, middle) 10 µm; (A, insets) 1 µm; (B) 5 µm.

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