Figure 5. Requirements for ARH targeting... | Rockefeller University Press

Figure 5.

$Figure 5. Requirements for ARH targeting to CCPs. BSC1 cells stably expressing ARH F259A-mCherry (A and B) or ARH S117Y;F165A-mCherry (C) were left untreated (A) or infected with adenoviruses coding for CD8/LDLR (B and C). Cells were then fixed, permeabilized, immunolabeled as indicated, and observed by epifluorescence. Bars, 10 µm. (D) Indicated cell lines were cultured as previously described and analyzed by Western blot after subcellular fractionation. S: cytosolic fraction (diluted 4x); P: membrane fraction. Actin served as loading control. The cell fractionation experiment has been repeated at least three times and a typical experiment is shown.$

Requirements for ARH targeting to CCPs. BSC1 cells stably expressing ARH F259A-mCherry (A and B) or ARH S117Y;F165A-mCherry (C) were left untreated (A) or infected with adenoviruses coding for CD8/LDLR (B and C). Cells were then fixed, permeabilized, immunolabeled as indicated, and observed by epifluorescence. Bars, 10 µm. (D) Indicated cell lines were cultured as previously described and analyzed by Western blot after subcellular fractionation. S: cytosolic fraction (diluted 4x); P: membrane fraction. Actin served as loading control. The cell fractionation experiment has been repeated at least three times and a typical experiment is shown.