ARH localization is cargo dependent. BSC1 cells stably expressing ARH-mCherry were infected with adenoviruses coding for CD8/LDLR and cultured in the presence (A and B) or absence (C and D) of tet. Cells were then fixed, permeabilized, immunolabeled as indicated, and observed by epifluorescence. Insets show enlarged areas. (E) BSC1 cells coexpressing Dab2-mCherry, ARH-EGFP, and CD8/LDLR were fixed and visualized by spinning disk confocal microscopy. Bars, 10 µm.