Requirements for Dab2/LDLR-induced GCCS formation. BSC1-wt cells were transfected with either myristoylated Dab2-mCherry (A) or Dab2-p67-mCherry (B and C) and were infected with adenoviruses coding for CD8/LDLR as indicated. Cells were fixed, permeabilized, immunolabeled in green for clathrin heavy chain (CHC; B and C), and observed by epifluorescence. Bars, 10 µm.