Depolymerases set oscillation and breathing speeds, whereas sister linkage modulates oscillation and breathing period. (a and h) Sister separation. (b and i) Sister center normal and projection speeds and sister breathing speed. (c–f, j, and k) Sister displacement cross-correlation and sister center autocorrelation (c, e, and k) and sister breathing autocorrelation (d, f, and j) are shown. Line thickness represents the 95% confidence interval of the correlations about their calculated values (see Materials and methods). Note that all experiments in e and f were performed at the University of Dundee (Dundee, Scotland, UK), not at the Marine Biological Laboratory (Woods Hole, MA). These different experimental settings led to small but highly reproducible changes in the oscillation and breathing periods of WT cells. Therefore, cell perturbations were always compared with the control data acquired at the same site. (g) Sister displacement cross-correlation at lag ΔT = 0. ns, not significant. Error bars indicate mean ± SD, representing cell to cell variation. Green and red double asterisks highlight values significantly larger and smaller than WT, respectively, unless indicated otherwise (i.e., P < 0.001 by one-tailed t test).