Nonrandom sister oscillation and breathing require bipolar attachment of kinetochores to dynamic MT plus ends. (a) Sister separation. (b) Sister center normal and projection speeds and sister breathing speed. (c–f) Sister displacement cross-correlation (c and e), sister center autocorrelation (c), and sister breathing autocorrelation (d and f) calculated for each condition by combining all aligned sister kinetochores belonging to that condition. Line thickness represents the 95% confidence interval of the correlations about their calculated values (see Materials and methods). S, synchronization. Error bars indicate mean ± SD, representing cell to cell variation. Green and red double asterisks highlight values significantly larger and smaller than WT, respectively, unless indicated otherwise (i.e., P < 0.001 by one-tailed t test).