Localization and function of Wee1 kinases in meiotic arrest. (A and D) Oocytes were injected with the indicated Wee1B (A) or Myt1 (D) mRNAs, washed out of the inhibitor, and monitored for the absence of a GV (GVBD). These experiments were repeated three times, and the error bars indicate SEM. The expression levels of injected mRNAs were compared by immunoblotting with the GFP antibody. (B) The activity of Wee1B-targeting mutants was measured by H1 kinase assay. The data are representative of three independent experiments. The ratio of 32P incorporation into histone H1 and the amount of immunocomplexes were densitometrically measured and represented as a mean percentage ± SEM of control (Mock). (C) Localization of WT and NLS mutants of Myt1 was shown with brightfield and fluorescence images. Bar, 20 µm.
