Nucleocytoplasmic shuttling of Wee1B is mediated by NLS and NES. (A) Schematic representation of the structure of Wee1B. The catalytic domain is shaded. The potential NLS sequence is indicated by a blue bar and aligned with human homologues. The putative NES sequence is indicated by a red bar and shown with the other known NES sequences. The consensus sequences of NLS and NES are shown in bold. The numbers above the structure refer to the amino acids of Wee1B. (B) Localization of Wee1B-targeting mutants was shown with representative brightfield and fluorescence images. (C) Immunoprecipitation (IP) of Wee1B-targeting mutants and Crm1. WB, Western blot. Bar, 20 µm.