Figure 3. Gliding assays with purified... | Rockefeller University Press

Figure 3.

$Figure 3. Gliding assays with purified Ncd and HL-KLP61F or KLP61F stalk: stochastic force-balance model that includes motors’ kinetics accounts for the results, but a protein friction model does not. (A) Competitive gliding assay results for Ncd with HL-KLP61F (left) and KLP61F stalk (right) for various mole fractions of Ncd, ranging from 0 to 1. (B) Results of stochastic, opposing power stroke force-balance model (red) including the kinetics of individual motors superimposed on experimental results (black). (A and B) Error bars show SD. (C) Histogram of distances MTs travel at the balance point mole fraction (∼0.7; in gliding assays shown in A) and the Gaussian distribution fit (red).$

Gliding assays with purified Ncd and HL-KLP61F or KLP61F stalk: stochastic force-balance model that includes motors’ kinetics accounts for the results, but a protein friction model does not. (A) Competitive gliding assay results for Ncd with HL-KLP61F (left) and KLP61F stalk (right) for various mole fractions of Ncd, ranging from 0 to 1. (B) Results of stochastic, opposing power stroke force-balance model (red) including the kinetics of individual motors superimposed on experimental results (black). (A and B) Error bars show SD. (C) Histogram of distances MTs travel at the balance point mole fraction (∼0.7; in gliding assays shown in A) and the Gaussian distribution fit (red).