KLHL21 localizes to the midzone and targets Cul3 to this region. (A) HeLa cells pre-extracted before fixation were analyzed by IF for KLHL21, Cul3, KLHL9, and KLHL13 and α-tubulin. Boxes in the telophase rows outline areas magnified in the panels below. (B and C) Cells treated with the indicated siRNAs were analyzed by IF for Cul3 (B) or KLHL21 (C) and α-tubulin. DNA was stained with DAPI. Bars, 5 µm.