KLHL21 is a novel regulator of the CPC. (A and B) HeLa cells treated with the indicated siRNAs were analyzed by IF for Aurora B and centromeres (A) or survivin (B). DNA was stained with DAPI. (C) Quantification of the relative intensity of Aurora B from A. The intensity on the midzone was divided by the intensity on anaphase chromosomes (n = 20). (D) HeLa cells treated with control or KLHL21 siRNA were analyzed by IF for Aurora B and Ser10-phosphorylated histone H3 (PH3). Quantification of the relative intensity of Ser10-phosphorylated histone H3 is shown. The intensity on anaphase chromosomes was divided by the intensity on metaphase chromosomes (n = 20). (C and D) Bars represent the mean with standard deviation of three independent experiments. Bars, 5 µm.