Histone H3S10 phosphorylation in mouse preimplantation embryos. (A) Time-lapse images of an embryo injected with 100 µg/ml Fab311-488 and 5 µg/ml H2B-mRFP mRNA (maximum projection of 51 z stacks). Arrows indicate the concentration of Fab311 in the centromere-rich regions. See Video 4. (B) Maternal chromosomes are selectively detected by Fab313. Fab313-488 (red) and H2B-mRFP (cyan) images in first and second mitosis are shown. The two-cell image is contrast enhanced. See Video 5. (C) Pups developed from Fab313-488–injected embryos with their recipient mother. (D) Development of embryos injected with fluorescent Fab. Embryos generated by in vitro fertilization were injected with various concentrations (conc.) of Fab together with H2B-mRFP mRNA, imaged up to the four to eight-cell stage, and transferred to the oviduct of day 0.5 pseudopregnant mothers. The rates of normal development were similar to controls (Yamagata et al., 2009). ^aImaging minus (−) means the embryos were injected with Fab but not imaged. Bars, 10 µm.