Aki1 localizes to centrosomes. (A) HeLa cells were costained for Aki1 (green) and centrin2 (red). Interphase or mitotic cells are shown. Arrows indicate the position of centrosomes, and insets show high magnification images of centrosomes. (B) HeLa cells stably expressing AcGFP-Aki1 (green) were counterstained for γ-tubulin (γ-tub; red). (C) Immunoblots were performed on cytoplasmic, nuclear, and sucrose gradient fractions (centrosome preparations) from HeLa cells with the indicated antibodies. Bars, 5 µm.