Cyclin B degradation profiles reflect the mitotic timing in bubR1-KEN and double mutant bubR1-KEN mad2 neuroblasts. Typical degradation profiles of GFP–cyclin B disappearance in single neuroblasts. (A–E) WT (A), mad2 (B), bubR1-KD mad2 (C), bubR1-KEN (D), and bubR1-KEN mad2 (E). The OCBD is unaffected by bubR1-KEN, beginning ∼6 min after NEB and 2–3 min before anaphase, as in WT. (F) Mean time of OCBD versus NEB (left) and anaphase (ANA) onset (right). OCBD begins 1.6 min earlier in mad2 or bubR1-KD mad2 mutant cells but 3 min earlier in bubR1-KEN mad2 mutant cells. Error bars indicate SD. See Videos 8–10 and Buffin et al. (2007). Bar, 5 µm.