Figure 1.
Figure 1. In vivo FLIM–FRET assay for chromatin compaction. (A) HeLa cells coexpressing either free EGFP and mCherry (top) or EGFP-fused to mCherry through a 7-aa linker (bottom) were imaged by multiphoton laser-scanning microscopy (MPLSM). The spatial distribution of the mean fluorescence lifetime of the EGFP donor (τ map) is displayed using a continuous pseudocolor scale ranging from 1.8 to 2.4 ns. (B) Representation of the FLIM–FRET chromatin compaction assay. Bars, 10 µm.

In vivo FLIM–FRET assay for chromatin compaction. (A) HeLa cells coexpressing either free EGFP and mCherry (top) or EGFP-fused to mCherry through a 7-aa linker (bottom) were imaged by multiphoton laser-scanning microscopy (MPLSM). The spatial distribution of the mean fluorescence lifetime of the EGFP donor (τ map) is displayed using a continuous pseudocolor scale ranging from 1.8 to 2.4 ns. (B) Representation of the FLIM–FRET chromatin compaction assay. Bars, 10 µm.

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