Lte1 maintains apical localization of the polarity cap through a MEN-independent mechanism. (A) The polarity caps of MGY308 wild type, MGY309 lte1Δ, and MGY470 lte1Δ bfa1Δ cells with medium-sized buds were visualized using Spa2- and Kel1-GFP. (B) Quantification of wild-type (WT; n = 70), lte1Δ (n = 100), and lte1Δ bfa1Δ (n = 100) medium-sized budding cells cultivated at 30°C and lte1Δ (n = 100) and lte1Δ kel1Δ (n = 100) cells cultivated at 12°C with apical (a), lateral (l), or dispersed (d) Kel1- and/or Spa2-GFP. (C) Bfa1 and tubulin were visualized in SY161 lte1Δ kel1Δ by indirect immunofluorescence after overnight growth at 12°C. Open arrowheads indicate SPBs where Bfa1 is absent. Closed arrowheads indicate SPBs with Bfa1. Cells (n = 100) were scored for asymmetric (85%) or symmetric (15%) localization of Bfa1. Bars, 5 µm.