Expression of Lte1 in the mother increases the asymmetric SPB localization of Bfa1 on misaligned spindles. (A) Bfa1, tubulin, and nuclei were monitored in binucleate mother cells with elongated spindles after overnight growth at 14°C. MGY407 (LTE1 dyn1) cells are shown in the top panels, and MGY408 (lte1-8N dyn1) cells are shown in the bottom panels. Open arrowheads indicate SPBs where Bfa1 signal is diminished. Closed arrowheads indicate SPBs with Bfa1. Cells are outlined in white. (B) The ratios of Bfa1 signal intensities were recorded in binucleate cells observed in A (n > 50). Bar, 5 µm.