Lte1 is required for asymmetric localization of Bfa1 at dSPBs at 12°C. (A) Cells were grown overnight at 12°C. Bfa1 and tubulin were monitored by indirect immunofluorescence in wild-type 15D, SY144 lte1Δ, and MGY389 bfa1Δ cells with extended anaphase spindles. Open arrowheads indicate SPBs where Bfa1 is absent. Closed arrowheads indicate SPBs with Bfa1. Cells are outlined in white. (B) The percentage of wild-type or lte1Δ cells cultivated at 30 or 12°C in late anaphase with Bfa1 at 0, 1, or 2 SPBs was determined by the presence of Bfa1 signals at the ends of the elongated spindle (n > 100). Bar, 5 µm.