Lte1 activity in vivo requires multiple domains. (A) Subcellular localization of Lte1-ΔEcoRI–GFP expressed from pMG158 in lte1Δ SY144. (B) Complementation of cold sensitivity of the lte1Δ strain SY144 with wild-type (WT) or ΔEcoRI forms of Lte1 expressed from the integrative plasmid pMG157. (C) Complementation of synthetic lethality of lte1Δ spo12Δ strain SY169 by Lte1 and Lte1-ΔEcoRI. The lte1Δ spo12Δ strain carried a URA3-based plasmid expressing wild-type LTE1. After transformation with integrative plasmids expressing Lte1 (pMG52) or Lte1-ΔEcoR1 (pMG157), cells were tested for growth on fluoroorotic acid (FOA) plates. YPD, YEP dextrose. Bar, 5 µm.