Rttn localizes to the centrosome and basal bodies. (A) A metaphase HeLa cell stained for Rttn (green), γ-tubulin (red), and DNA (blue). Rttn antibodies costain with γ-tubulin at the centrosome but label a smaller dot, suggesting that Rttn localizes to centrioles. (B) An interphase HB2 cell stained for Rttn (green) and acetylated tubulin (red). Rttn antibodies stain the basal bodies at the base of the cilium marked by acetylated tubulin. Bars, 10 µm.