Ana3 is a component of centrioles and basal bodies, but its localization is distinct from the core duplication proteins. (A) A syncytial embryo expressing Ana3-GFP (green) stained for tubulin (red) and DNA (blue). Ana3-GFP localizes to centrosomes throughout the cell cycle, which is shown at metaphase. (B) Interphase brain cells expressing Ana3-GFP (green) stained for the centriole marker Asl (red) and DNA (blue). Ana3-GFP localizes to centrosomes throughout the cell cycle, indicating that Ana3 is a centriolar component. (C) A large, v-shaped centriole pair from a primary spermatocyte expressing Ana3-GFP (green) and DSas-4–RFP (red). Ana3-GFP is distributed evenly along the centriole barrels. In contrast, DSas-4–RFP, like the other conserved core duplication proteins, is enriched at the proximal and distal ends of the centrioles. (D) Spermatids expressing Ana3-GFP (green) stained for DNA (blue). Ana3-GFP localizes to the basal bodies attached to the elongating nuclei. Bars: (A, B, and D) 10 µm; (C) 2 µm.