Figure 3.
Merotelic attachment influences the rate of spindle elongation. (A) Kymograph of a rad21-K1 ndc80-gfp cdc11-cfp cell showing a single, stretched Kt. (B) Spindle elongation rate of rad21-K1 cdc11-cfp ndc80-gfp cells in the absence of merotelic attachment (zero) or in presence of one, two, or three merotelic Kts. (C) Graphic representation of spindle elongation in the rad21-K1 ndc80-gfp cdc11-gfp cell shown in A (red dots). The rate of spindle elongation was calculated before (red) or after (blue) disruption of the merotelic Kt. Error bars indicate SD.