Figure 2.
Kt stretching is imposed by MT-dependent forces. (A) A mixed population of atb2-gfp cdc11-cfp (control) and rad21-K1 ndc80gfp cdc11-cfp cells (merotelic Kt) was treated simultaneously by laser ablation. Image series showing the two cells before (time 0) and at the moment of laser ablation (see Materials and methods). Schematic representations of fission yeast cell shape are shown (dashed ovals). (B) Kymograph of the rad21-K1 cell shown in A illustrating the relaxation of the merotelic Kt after laser ablation. Blue dots indicate the point of laser impact.