Figure 5.
Figure 5. Telomeres are released from the NE in bqt4Δ cells. (A and B) Cells expressing Taz1-mCherry (magenta) and Ccr1N-GFP (green) were observed during mitosis (A) and meiosis (B). Numbers on the left of each image in B indicate the time in minutes. The image at each time point is a projection of three optical sections around the nuclear midline taken in three dimensions. The strains observed are CRLr75 (wild type), CRLr81 (bqt4Δ), and CRLr82 (bqt2Δ). Percentile ranks of the distance between the telomere and the NE are shown in the right panels. The vertical axis is the 3D radial distance D for mitotic cells and is the 2D in-focus distance d for meiotic cells (see Fig. 9 and Materials and methods). In a percentile rank plot, the percentage of telomeres in which the distance to the NE is equal or less than a given distance is plotted as a function of the distance. Bars: (A) 1 µm; (B) 10 µm.

Telomeres are released from the NE in bqt4Δ cells. (A and B) Cells expressing Taz1-mCherry (magenta) and Ccr1N-GFP (green) were observed during mitosis (A) and meiosis (B). Numbers on the left of each image in B indicate the time in minutes. The image at each time point is a projection of three optical sections around the nuclear midline taken in three dimensions. The strains observed are CRLr75 (wild type), CRLr81 (bqt4Δ), and CRLr82 (bqt2Δ). Percentile ranks of the distance between the telomere and the NE are shown in the right panels. The vertical axis is the 3D radial distance D for mitotic cells and is the 2D in-focus distance d for meiotic cells (see Fig. 9 and Materials and methods). In a percentile rank plot, the percentage of telomeres in which the distance to the NE is equal or less than a given distance is plotted as a function of the distance. Bars: (A) 1 µm; (B) 10 µm.

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