Localization of Bqt3 and -4 to the INM. (A) Localization of Bqt4 and -3 proteins in vegetatively growing cells, as determined by immunoelectron microscopy. Arrowheads indicate GFP-Bqt4 or -Bqt3, in which GFP was fused to the N terminus of Bqt4 or -3. Samples were stained with polyclonal anti-GFP and immunogold-conjugated secondary antibodies. The observed strains are CRLr06 (h⁻ bqt4Δ::ura4⁺, lys1::GFP-bqt4) and CRLs86 (h⁻ bqt3Δ::LEU2, lys1::GFP-bqt3). (B) Localization of GFP-Bqt4 and –Bqt4-dTM in vegetatively growing cells. The observed strains are CRLt53 (left; h⁻ bqt4Δ::ura4⁺, lys1::GFP-bqt4) and CRLt54 (right; h⁻ bqt4Δ::ura4⁺, lys1::GFP–bqt4-dTM). (C) Expression of Bqt4-dTM protein did not suppress the loss of telomere clustering. Cells expressing both Taz1-mRFP (red) and GFP-Bqt4 or –Bqt4-dTM (green) were observed after the induction of meiosis. The observed strains are CRLs53 (h⁹⁰ bqt4Δ::ura4⁺, lys1::GFP-bqt4, taz1-mCherry::kan) and CRLs38 (h⁹⁰ bqt4Δ::ura4⁺, lys1::GFP–bqt4-dTM, taz1-mCherry::kan). Bars: (A) 200 nm; (B and C) 10 µm.