DIAP1 dynamics depend on cell type. In numb2 mutant clones, PRAP dynamics changed with cells’ transformation to a new fate. (A–F) The PRAP pattern was dependent on cell fate. PRAP accumulation was observed in all four cells after cell division, and it was degraded only in the shaft cell (E and F, arrowheads). The final fate of the cells was confirmed by examining the outer morphology, after the imaging analysis. The numb2 mutant clone is marked by Histone2B-ECFP (magenta) and PRAP (green) expression. Dashed lines outline the nuclei. (G–J) Merged images of cross sections shown in E and F. Bar, 5 µm. (K) The numb2 mutant causes the transformation of a pIIb cell into an ectopic pIIa cell. The genotype was Ubx-flp/+; y+ numb2 ck FRT40A/tub-GAL80 FRT40A; neu-GAL4 UAS-Histone2B-ECFP/UAS-PRAP22.
