A candidate Y. lipolytica Inp2p orthologue, YALI0E03124p, does not localize to peroxisomes. The chimeric protein YALI0E03124p-mRFP, whose expression is under the control of the oleic acid–inducible promoter POT1, was imaged in the wild-type strain E122 expressing genomically integrated Pot1p-GFP to fluorescently label peroxisomes. YALI0E03124p-mRFP did not localize to punctate peroxisomes, and when cells were incubated in oleic acid–containing medium, YALI0E03124p-mRFP exhibited a pattern typical of protein localization to the ER and secretory system. The top panels show representative images of cells grown in medium containing acetate, whereas the bottom panels show representative images of cells grown in medium containing oleic acid. Bar, 5 µm.