Deletion of the PEX3B gene affects peroxisome inheritance. (A) Wild-type and pex3BΔ cells expressing genomically integrated POT1-GFP were grown in YPD for 16 h and then transferred to YPBO for 2 h. Fluorescent images of randomly chosen fields of cells were acquired as a stack by confocal microcopy and deconvolved. Buds were sized according to four categories relative to the volume of the mother cell (see Materials and methods). The percentages of bud tips containing peroxisomes at each size category were plotted. Quantification was performed on at least 50 budded cells from each category. Graphic results are the means and SEM of three independent experiments. Bar, 5 µm. (B) Deletion of the PEX3B gene does not affect the actin structure of cells or the inheritance of vacuoles or mitochondria. Wild-type and pex3BΔ cells synthesizing Pot1p-GFP were grown in YPD. Mitochondria were stained with Mitotracker dye, vacuoles were stained with the fluorophore FM4-64, and actin was stained with rhodamine-phalloidin. Images were captured by confocal microscopy. Bar, 5 µm.