Figure 3.
Figure 3. Deletion of the PEX3B gene affects peroxisome morphology. (A) Wild-type and pex3BΔ cells expressing genomically integrated POT1-GFP were grown in glucose-containing YPD for 16 h and then transferred to oleic acid–containing YPBO. Fluorescent images of cells at different times of incubation in YPBO were captured by confocal microscopy and deconvolved. Bar, 5 µm. (B) Cells lacking Pex3Bp contain elongated peroxisomes. An elongated peroxisome was functionally defined as being 2 µm or greater in length along its long axis. Graphic results are the means and SEM of three independent experiments. (C) Ultrastructure of wild-type E122 and pex3BΔ cells. Cells were cultured in YPD for 16 h, transferred to YPBO for 10 h, and then fixed and processed for EM. Arrowheads indicate Individual peroxisomes. Bar, 1 µm. (D) Tracings of individual peroxisomes in the electron micrographs of cells presented in C. Bar, 1 µm.

Deletion of the PEX3B gene affects peroxisome morphology. (A) Wild-type and pex3BΔ cells expressing genomically integrated POT1-GFP were grown in glucose-containing YPD for 16 h and then transferred to oleic acid–containing YPBO. Fluorescent images of cells at different times of incubation in YPBO were captured by confocal microscopy and deconvolved. Bar, 5 µm. (B) Cells lacking Pex3Bp contain elongated peroxisomes. An elongated peroxisome was functionally defined as being 2 µm or greater in length along its long axis. Graphic results are the means and SEM of three independent experiments. (C) Ultrastructure of wild-type E122 and pex3BΔ cells. Cells were cultured in YPD for 16 h, transferred to YPBO for 10 h, and then fixed and processed for EM. Arrowheads indicate Individual peroxisomes. Bar, 1 µm. (D) Tracings of individual peroxisomes in the electron micrographs of cells presented in C. Bar, 1 µm.

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