Myosin VI–coated nanosphere movement on extracted keratocyte actin networks does not result in or require actin filament reorganization. (a, left) Platinum replica micrograph of an extracted keratocyte prepared after movement of myosin VI artificial dimer-coated nanospheres on its surface. (a, middle) A higher resolution image showing the size of nanospheres relative to the F-actin network size. (a, right) A schematic diagram depicting the relative sizes of a nanosphere (highlighted by the green circle in the center and right panels), the myosin VI dimers, and the keratocyte actin network. The right panel shows two dimers bound to actin filaments, coupled through the nanosphere (green line), and bound to the nanosphere at the yellow circles. (b) Trajectories of movement of myosin VI dimer-coated nanospheres on a keratocyte extracted and fixed with 2% formaldehyde for 10 min. (c) Sample trajectories from panel b without exception show movement toward the cell center. Bars, 1 µm.