Figure 1.
Figure 1. Drp1 is required for embryonic mouse development. (A) Design of Drp1 knockout alleles. WT, wild type. (B) Confirmation of knockout by PCR. Genomic DNAs were prepared from embryos, yolk sack, or tails of adult mice for PCR. To distinguish Drp1+/+, Drp1+/−, and Drp1−/−, D1, D3, and D5 were used. Wild-type and null alleles produced 315-bp and 539-bp PCR products, respectively. For floxed alleles, D3 and D6 were used. Wild-type (+) and floxed (F) alleles generated 355-bp and 532-bp PCR products, respectively. (C) Genotypes of offspring from breeding of Drp1+/− mice. (D) Number of viable embryos. (E) Immunoblotting of embryos. (F) Images of embryos. (G) Crown rump length of embryos. ***, P < 0.001 (n ≥ 14).

Drp1 is required for embryonic mouse development. (A) Design of Drp1 knockout alleles. WT, wild type. (B) Confirmation of knockout by PCR. Genomic DNAs were prepared from embryos, yolk sack, or tails of adult mice for PCR. To distinguish Drp1+/+, Drp1+/−, and Drp1−/−, D1, D3, and D5 were used. Wild-type and null alleles produced 315-bp and 539-bp PCR products, respectively. For floxed alleles, D3 and D6 were used. Wild-type (+) and floxed (F) alleles generated 355-bp and 532-bp PCR products, respectively. (C) Genotypes of offspring from breeding of Drp1+/− mice. (D) Number of viable embryos. (E) Immunoblotting of embryos. (F) Images of embryos. (G) Crown rump length of embryos. ***, P < 0.001 (n ≥ 14).

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