Transmembrane cargo is lost from maturing DCVs in the absence of Rab2. (A and B) Representative images of IDA-1–GFP in DA/DB motor neuron axons in wild type (A) and unc-108(nu415) (B). Images are scaled identically for brightness after adjusting for differences in light source power. IDA-1–GFP is expressed from the integrated transgenic array ceIs72. (C) Quantification of IDA-1–GFP fluorescence in the dorsal axons of wild type and the unc-108 mutant. The graph shows the total integrated fluorescence per micrometer (arbitrary fluorescence units [a.f.u.]) of dorsal axon length in the indicated backgrounds. (D and E) Representative images of IDA-1–GFP in DA6/DB6 motor neuron cell somas in wild type (D) and unc-108(nu415) (E). Images are scaled identically for brightness after adjusting for differences in light source power. (F) Quantification of IDA-1–GFP fluorescence in the DA6/DB6 cell somas of wild type and the unc-108 mutant. The graph shows the total integrated fluorescence per square micrometer (arbitrary fluorescence units) of cell soma and dendrite area in the indicated backgrounds. (C and F) Data are means and SEMs from 14 animals for each strain. The p-values are from the Mann-Whitney test.