Blocking neuropeptide processing or PI(3)P restores normal levels of the Venus cargo in unc-108 mutant cell somas. (A–F) Representative images of NLP-21–Venus in DA6/DB6 motor neuron cell somas in animals with the indicated genetic backgrounds. Strains containing the same transgenic array are scaled identically for brightness after adjusting for differences in light source power. NLP-21–Venus is expressed from the integrated transgenic array ceIs56 in A–D and ceIs57 in E and F. DA6 and DB6 neurons show variable spacing. The process between the somas is a dendrite, not an axon. (A and B) UNC-108 is required for normal levels of the Venus cargo in cell somas. (C and D) Blocking peptide processing with an egl-3 (PC2 convertase) null mutation restores near normal levels of the Venus cargo in the cell somas of unc-108 null mutants. (E and F) Blocking PI(3)P function with excess 2XFYVE restores normal levels of the Venus cargo in the cell somas of wild type and unc-108 null mutants. (G) Quantification of Venus cargo levels in the cell somas of unc-108 mutants. The graph shows the total integrated fluorescence per square micrometer of cell soma and dendrite area, expressed as arbitrary fluorescence units (a.f.u.). Data are means and SEMs from 10 to 15 individual animals each. P-values are from a Mann-Whitney test.